Evaluation of VIDAS® Diagnostic Assay Prototypes Detecting Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM and IgG Antibodies

Dengue is a serious tropical disease caused by the mosquito-borne dengue virus (DENV). Performant, rapid, and easy-to-use assays are needed for accurate diagnosis of acute DENV infection. We evaluated performance three prototype developed VIDAS® automated platform to detect NS1 antigen anti-dengue IgM IgG antibodies. Positive negative agreement with competitor enzyme-linked immunosorbent (ELISA) rapid diagnostic tests (RDT) was in 91 Lao patients (57 adults, 34 children) The assay showed best overall (95.6%) ELISA. Both ELISA also demonstrated high sensitivity relative RNA RT-PCR set as gold standard (85.7% 83.9%, respectively). In contrast, RDT less sensitive (72.7%). moderate (72.5% ELISA, 76.9% 68.7% RDT). most analyses, test agreements were comparable adults children. Altogether, prototypes performed very well appear be suitable routine detection IgM/IgG